Size-exclusion chromatography (SEC), also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight. It is usually applied to large molecules or macromolecular complexes such as proteins and industrial polymers. We often hear the terms 'size exclusion chromatography' and 'GPC', but let's briefly review some basics to find out what sort of information they provide. Size exclusion chromatography (SEC), also known as gel filtration, is the simplest and mildest of all the chromatography techniques. SEC separates molecules by differences in size as they pass through a SEC resin packed in a column. 15 de nov de 2013 - SIZE EXCLUSION CHROMATOGRAPHY Presented by MANDAWI TRIPATHI. 18-1115-69. Size Exclusion Chromatography. Principles and Methods. GE Healthcare. Life Sciences. Size Exclusion. Chromatography. Principles and Methods. Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel. Size exclusion chromatography (SEC) separates molecules based on their size by filtration through a gel. The gel consists of spherical beads containing pores . 30 de nov de 2012 - This need has renewed interest in size-exclusion chromatography (SEC). In the following review we will outline the history and background of .